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| Registros recuperados: 237 | |
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| Aragão,Annelize Zambon Barbosa; Teocchi,Marcelo Ananias; Fregolente,Maria Clara Duarte; Gatti,Maria Silvia Viccari; Pires,Alexandre Vaz; Yano,Tomomasa. |
| Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Lambs; STa; Colibacillosis; Escherichia coli. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000500015 |
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| Pinheiro Jr.,A.A.; Oliveira,L.A.T.; Franco,R.M.; Carvalho,J.C.A.P.. |
| Analisaram-se amostras de água do mar de cinco pontos da Baía de Guanabara quanto ao número mais provável (NMP) de coliformes totais e de Escherichia coli usando-se o método fluorogênico, e verificaram-se o pH, a salinidade e a temperatura dos locais de colheita. As contagens de E. coli nas amostras das estações Icaraí, Boa Viagem e Santa Cruz foram, em média, acima do previsto na legislação, enquanto que nas estações Piratininga e Rio Branco, 92,9% e 100% das amostras tiveram contagens de E. coli menor que 3/100ml. Observou-se relação positiva entre coliformes totais e E. coli, enquanto que a correlação entre colimetria, pH, salinidade e temperatura da água não foi significativa. Temperatura e pH mostraram pouca variação entre as estações, sendo as médias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coliformes; Escherichia coli; Águas de cultivo; Baía de Guanabara. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400016 |
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| Plucain, Jessica; Suau, Antonia; Cruveiller, Stephane; Medigue, Claudine; Schneider, Dominique; Le Gac, Mickael. |
| Background The impact of historical contingency, i.e. the past evolutionary history of a population, on further adaptation is mostly unknown at both the phenotypic and genomic levels. We addressed this question using a two-step evolution experiment. First, replicate populations of Escherichia coli were propagated in four different environmental conditions for 1000 generations. Then, all replicate populations were transferred and propagated for further 1000 generations to a single new environment. Results Using this two-step experimental evolution strategy, we investigated, at both the phenotypic and genomic levels, whether and how adaptation in the initial historical environments impacted evolutionary trajectories in a new environment. We showed that... |
| Tipo: Text |
Palavras-chave: Experimental evolution; Escherichia coli; Adaptation; Historical contingency; Epistasis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00333/44447/44118.pdf |
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| Bialvaei,Abed Zahedi; Kafil,Hossein Samadi; Asgharzadeh,Mohammad; Aghazadeh,Mohammad; Yousefi,Mehdi. |
| Abstract This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLs producing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins. After identification and susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evaluated for ESBL production by the disk combination method and by Etest using (cefotaxime and cefotaxime plus clavulanic acid). All isolates were also screened for bla CTX-M using conventional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonella spp and Shigella... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella spp; Salmonella spp; Shigella spp; Escherichia coli; Multi-drug resistant; ESBL; CTX-M. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300706 |
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| Derrien, Annick. |
| Le rôle des sédiments dans la contamination du milieu marin en baie de l'Aiguillon est une des problématiques du laboratoire. Pendant quinze mois des dénombrements d'Escherichia coli dans des sédiments de la baie ont été effectués. Deux méthodes de numération ont été comparées: la technique NPP en tubes et la technique NPP en microplaque. En période printemps-été, la méthode NPP miniaturisée a conduit à des résultats sensiblement supérieurs à ceux obtenus par la méthode NPP en tubes. Cette différence pourrait s'expliquer par une fluorescence parasite du sédiment pendant cette période (éventuellement par du plancton; hypothèse non confirmée). Les concentrations en E.coli des sédiments sont variables au cours de l'année avec un niveau de contamination faible... |
| Tipo: Text |
Palavras-chave: Sédiments; Escherichia coli; Dénombrement. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00107/21811/19386.pdf |
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| Díaz,Mauricio; Arenas,Gloria; Marshall,Sergio H. |
| Novel doublet molecules of cecropin A from Drosophila melanogaster were designed and constructed combining the regular (CECdir) with the inverted (CECret) coding sequence of the standard CEC A1 gene resulting in the following configurations: CECdir-CECret and CECret-CECdir. These two recombinant molecules were generated using a three-primer driven PCR reaction yielding composite single functional aminoacidic molecules with the coding sequences of CECdir linked in frame with the coding sequence of CECret and vice versa. In order to obtain these constructions, a retropeptide DNA-coding sequence was chemically synthesized to match the expected polarity of the newly generated CECret sequence. Both doublet antimicrobial peptides (drAMPs) were cloned in the T7... |
| Tipo: Journal article |
Palavras-chave: Antimicrobial peptides; Escherichia coli; Expression. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200006 |
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| Macêdo,N.R.; Menezes,C.P.L.; Lage,A.P.; Ristow,L.E.; Reis,A.; Guedes,R.M.C.. |
| Avaliou-se a freqüência dos genes de fímbrias (K88, K99, 987P, F18 e F41) e toxinas (LT, Stb, StaP e Stx2e) de cepas de E. coli isoladas de leitões com diarréia usando a técnica de PCR multiplex com primers específicos para esses genes, e estudou-se o padrão de sensibilidade das cepas patogênicas pelo método de difusão em disco ao florfenicol, ceftiofur sódico, colistina, fosfomicina, neomicina, norfloxacina, sulfa + trimetoprim, doxiciclina, tetraciclina e lincomicina. Foram utilizadas 144 amostras de E.coli isoladas de leitões com diarréia, provenientes de granjas localizadas no estado de Minas Gerais. Dessas, 42 (29,2%) foram positivas para pelo menos um dos fatores de virulência testados. Dentre essas 42 amostras, 23 (54,8%) apresentaram genes de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Suíno; Escherichia coli; Tipificação; PCR multiplex; Sensibilidade antimicrobiana. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500005 |
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| Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.. |
| ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003 |
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| Otutumi,Luciana Kazue; Furlan,Antonio Claudio; Martins,Elias Nunes; Nakamura,Celso Vataru; Garcia,Elis Regina de Moraes; Loose,Priscila Ventura. |
| Objetivou-se avaliar duas vias de administração de probiótico sobre o desempenho, o rendimento de carcaça e a população microbiana do intestino delgado de codornas de corte. Foram utilizadas 1.050 codornas machos e fêmeas de 1 dia de idade distribuídas em delineamento inteiramente casualizado com três tratamentos (controle, probiótico na água bebida e probiótico na ração) e cinco repetições de 70 aves. A cada sete dias, foram avaliados o ganho de peso, o consumo de ração, a conversão alimentar e a viabilidade criatória. Aos 7 e aos 14 dias, 15 aves foram abatidas para determinação da contagem de Lactobacillus sp., enterobactérias e Escherichia coli do intestino delgado e, aos 35 dias, 30 aves foram abatidas para avaliação do rendimento de carcaça e de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conversão alimentar; Escherichia coli; Lactobacilos. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000100021 |
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| Registros recuperados: 237 | |
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